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5x reaction buffer suitable for use in most PCR applications, including real-time PCR. If it is necessary to select the concentration of Mg2+ ions in the reaction mixture, it is recommended to use PCR buffers that do not contain magnesium salts. The standard 5x PCR buffer contains 10 mm MgSO4, which corresponds to 2 mM MgSO4 in the reaction mixture.

The kit is based on multiplex amplification of 28 STR loci followed by separation of PCR products by capillary electrophoresis. Of these, 25 autosomal loci: D3S1358, D2S441, D1S1656, D19S433, TPOX, D2S1338, TH01, D16S539, CSF1PO, D18S51, D21S11, D22S1045, D8S1179, VWA, D5S818, FGA, D13S31 7, D7S820, D12S391, D10S1248, SE33, DYS391, D8S1132, D7S1517, Penta D, Penta E and 3 sex loci: Amelogenin, Yindel, DYS391. The STR loci presented in the set are highly polymorphic, which allows for reliable identification of a person. The set includes all loci from the CODIS (Combined DNA Index System) and ESS (European Standard Set) databases, as well as loci SE33, Penta D, Penta E. To obtain the complete (for all 28 STR loci) genotype of the test sample, 0.125 nanograms (ng) of non-degraded DNA is sufficient. The optimal amount of DNA introduced into the reaction is 1 ng. The range of DNA concentrations for amplification is from 0.1 to 4 ng per reaction.

The kit uses the technology of DNA sorption on magnetic particles. The entire DNA extraction process combines the steps of sample lysis, subsequent selective binding of the released DNA to magnetic beads, and then, after a series of washes, elution with the option of concentrating the DNA in a low-salt buffer.
Benefits of the set:

• High yield of genomic DNA from E.coli and eukaryotic cells: 4-5 µg DNA from 0.5-1*109 bacterial cells and 0.25-1*106 eukaryotic cells.
• High yield of genomic DNA when isolated from various mouse tissues and organs.
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Purified DNA is verified to be free of proteins, nucleases or other contaminants and can be used in a wide range of molecular biology techniques such as PCR, RT-PCR or other enzyme reactions.
• High throughput for DNA extraction from large numbers of samples.

a set of reagents for the secretion of nucleic acids (DNA/RNA) from biological material by sorption on magnetic particles. Compatible biomaterial: • Smears from the upper respiratory tract • Buccal epithelium • Saliva • Fecal extracts • Whole blood • Blood plasma

The dNTP mixture is an aqueous solution of four highly purified 2’-deoxynucleoside-5’-triphosphates (dATP, dTTP, dHTF, dCTF) in equimolar concentration

Genta-ExoI exonuclease I hydrolyzes single-stranded DNA in the 3'→ 5' direction, releasing deoxyribonucleoside 5'-monophosphate. It does not cleave DNA chains whose 3’-end is blocked by a phosphoryl or acetyl group. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the exonuclease I gene of E.coli.

The polymer provides single-nucleotide separation of fragments of the Sanger DNA sequencing reaction in the range from 20 to 1000 nucleotides as part of genetic analyzers operating on the principle of capillary gel electrophoresis, for example, Nanofor 05