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5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

Oligonucleotides are short fragments of nucleic acids that are widely used in various fields of DNA diagnostics (medical diagnostics, forensic analysis, veterinary medicine, food quality control, etc.). Recently, oligonucleotides have been widely used as medicines against various genetic and other diseases. GenTerra produces DNA and RNA oligonucleotides on a scale of several milligrams to gram quantities, including modified oligonuleotides, fluorescently labeled oligonucleotides and real-time PCR probes.

The kit is intended for the extraction and purification of DNA from the following samples: 1. Leaves, needles, stamens, green parts of plants 2. Roots, stems, bark 3. Fruits, berries, seeds 4. Mosses, lichens 5. Unicellular algae

The kit is based on multiplex amplification of 28 STR loci followed by separation of PCR products by capillary electrophoresis. Of these, 25 autosomal loci: D3S1358, D2S441, D1S1656, D19S433, TPOX, D2S1338, TH01, D16S539, CSF1PO, D18S51, D21S11, D22S1045, D8S1179, VWA, D5S818, FGA, D13S31 7, D7S820, D12S391, D10S1248, SE33, DYS391, D8S1132, D7S1517, Penta D, Penta E and 3 sex loci: Amelogenin, Yindel, DYS391. The STR loci presented in the set are highly polymorphic, which allows for reliable identification of a person. The set includes all loci from the CODIS (Combined DNA Index System) and ESS (European Standard Set) databases, as well as loci SE33, Penta D, Penta E. To obtain the complete (for all 28 STR loci) genotype of the test sample, 0.125 nanograms (ng) of non-degraded DNA is sufficient. The optimal amount of DNA introduced into the reaction is 1 ng. The range of DNA concentrations for amplification is from 0.1 to 4 ng per reaction.

5X Genta Taq-AB PCR Master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB PCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+ and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

Genta-ExoI exonuclease I hydrolyzes single-stranded DNA in the 3'→ 5' direction, releasing deoxyribonucleoside 5'-monophosphate. It does not cleave DNA chains whose 3’-end is blocked by a phosphoryl or acetyl group. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the exonuclease I gene of E.coli.