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Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

5X Genta Single-tube RT-qPCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-qPCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

ExoGen is a ready-to-use mixture of Genta-ExoI exonuclease I enzymes and the thermolabile alkaline phosphatase Genta-ALP in a reaction buffer. ExoGen mixture is used for enzymatic hydrolysis of an excessive amount of primers and nucleotides in PCR products (amplicons). The PCR products purified in this way are suitable for further use in various biological applications, such as cloning and DNA sequencing.

Medicines may contain pyrogens, the sources of which can be bacteria, viruses, fungi, and pyrogens can also be by-products of the production process. Contamination of drugs with pyrogenic substances is a serious threat to the health of patients and can lead to the development of fever and, ultimately, shock and death. Currently, to determine the content of pyrogenic substances (bacterial endotoxins and substances of non-endotoxin nature), the “Pyrogenicity” test is used, carried out on rabbits, which is inaccurate and harmful to animals. The monocyte activation test (MAT) has higher sensitivity and does not require the use of animals.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR

5X Genta Taq-AB PCR Master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB PCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+ and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

Fluorescent probes are an oligonucleotide with a fluorescent label and a suppressor that can bind to the DNA chain during amplification. When the probe is in a free state, both dyes are located close to each other, therefore, fluorescence quenching occurs. During PCR, at the stage of chain elongation, the hybridized probe is cleaved by a polymerase that has 5’-exonuclease activity, the dyes are separated, and a fluorescent signal proportional to the amount of the amplified product is observed. The fluorescence intensity increases in each cycle in proportion to the probe splitting rate. The use of probes in PCR makes it possible to increase the accuracy and selectivity of the reaction. A wide range of fluorescent dyes: FAM, R6G, VIC, HEX, JOE, TAMRA, ROX, Cy 5 and Cy5.5 The possibility of multiplex analysis of multiple DNA targets in one test tube. High efficiency of PCR-RV. Probe quality control: HPLC purification and mass spectrometry verification

Oligonucleotides are short fragments of nucleic acids that are widely used in various fields of DNA diagnostics (medical diagnostics, forensic analysis, veterinary medicine, food quality control, etc.). Recently, oligonucleotides have been widely used as medicines against various genetic and other diseases. GenTerra produces DNA and RNA oligonucleotides on a scale of several milligrams to gram quantities, including modified oligonuleotides, fluorescently labeled oligonucleotides and real-time PCR probes.

The kit “System for quantitative assessment of E. coli host DNA impurities by RT-PCR” is convenient and easy to use; it allows you to isolate residual DNA from a sample and amplify it using real-time PCR in the presence of the fluorescent dye SYBR Green I. The kit includes reagents for isolating DNA from nucleoprotein complexes and removing PCR inhibitors, a 2× reaction mixture BioMaster HS-qPCR SYBR Blue (2×), a standard DNA solution and a proven set of specific primers for the conserved region of E. coli 16S rRNA.

5X Genta Single-tube RT-PCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-PCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+ and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows for preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.