The kit is designed for isolating plasmid DNA from bacteria. The operating principle of the kit is based on alkaline lysis of RNase-treated biomaterial, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The colored solutions in the kit allow you to control the correct order of adding components, the completeness of lysis and neutralization. Benefits of the set:
Yield up to 25 µg from 2 ml of bacterial culture and up to 60* µg from 6-10 ml of bacterial culture
Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
Plasmid DNA has been verified to be suitable for restriction reactions, transformation, sequencing, PCR, transfection.
*product yield depends on the copy number of the plasmid, culture volume and cultivation conditions